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Dog Interleukin 2,IL-2 ELISA Kit

  • 中文名稱:
    犬白介素2(IL-2)酶聯免疫試劑盒
  • 貨號:
    CSB-E11258c
  • 規格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 其他:

產品詳情

  • 產品描述:
    犬白介素2(IL-2)酶聯免疫試劑盒(CSB-E11258c)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IL2含量。IL2是重要的免疫細胞因子,其背景在于它能促進T細胞等免疫細胞生長和分化。研究機制上,IL2與受體結合,激活下游信號通路,調控免疫細胞功能,增強機體免疫應答,在腫瘤免疫治療、自身免疫病研究等領域都是關鍵靶點。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,適用于獸醫科研領域對犬類免疫相關疾病的機制研究,如感染性疾病模型建立、疫苗免疫效果評估、炎癥反應動態監測等實驗場景。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    3.9 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays):CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 84
    Range % 81-89
    1:2 Average % 95
    Range % 92-98
    1:4 Average % 86
    Range % 83-90
    1:8 Average % 97
    Range % 94-100
  • 回收率:
    The recovery of dog IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 85 82-90
    EDTA plasma (n=4) 105 101-109
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.919 2.900 2.910 2.812
    500 2.424 2.468 2.446 2.348
    250 1.670 1.620 1.645 1.547
    125 0.981 0.967 0.974 0.876
    62.5 0.474 0.481 0.478 0.380
    31.2 0.289 0.276 0.283 0.185
    15.6 0.161 0.163 0.162 0.064
    0 0.099 0.097 0.098  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • 基因功能參考文獻:
    1. immunoreactivities of inflammatory cytokines, such as interleukin (IL)-2, its receptor (IL-2R), IL-4 and its receptor (IL-4R) in the cervical and lumbar spinal cord of young adult and aged beagle dogs, were compared. PMID: 23605681
    2. In dogs with leishmaniasis, there was high expression of genes encoding IL-2 in lymph nodes. PMID: 21511273
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    IL-2 family
  • 數據庫鏈接:

    KEGG: cfa:403989

    STRING: 9615.ENSCAFP00000005941

    UniGene: Cfa.7



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