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  4. Dog Interleukin 10,IL-10 ELISA Kit

Dog Interleukin 10,IL-10 ELISA Kit

  • 中文名稱：
    犬白介素10(IL-10)酶聯免疫試劑盒
  • 貨號：
    CSB-E11261c
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：
    犬白介素10(IL-10)酶聯免疫試劑盒（CSB-E11261c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL10含量。IL10是一種具有重要生物學意義的靶點。它是免疫調節細胞因子，具有抗炎特性。研究機制上，IL10可抑制促炎細胞因子產生，調節免疫細胞功能。對其深入研究有助于理解免疫相關疾病發病機制，為開發新治療方法提供方向。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，適用于犬類免疫機制研究、炎癥性疾病模型評估、疫苗或藥物干預效果分析等科研場景，為探索IL - 10在感染、腫瘤或自身免疫性疾病中的調控機制提供可靠數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    IL10 ELISA Kit; Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %97
    Range %92-108
    1:2Average %97
    Range %91-105
    1:4Average %89
    Range %84-96
    1:8Average %92
    Range %87-98
  • 回收率：
    The recovery of dog IL-10spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-95
    EDTA plasma (n=4)9287-96
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.954 2.967 2.961 2.853
    502.388 2.398 2.393 2.285
    251.758 1.786 1.772 1.664
    12.51.035 1.109 1.072 0.964
    6.250.502 0.546 0.524 0.416
    3.120.271 0.267 0.269 0.161
    1.560.184 0.196 0.190 0.082
    00.112 0.103 0.108 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

相關問答

■ 常見問題解答
Q:

If using this kit it is necessary to perform a a blood clotting time of 2 hours or overnight at 4 ° C and 15 minutes of centrifugation, and if so what is the reason for this procedure.

A:
Thanks for your inquiry.
It is the sample preparation method for serum indeed.
It is fine to do as the you mentioned for serum. Here also attached our manual for your checking.
The sample preparation method is through our our experimental experience and get it to prepare relatively large amounts of serum samples with relatively simple components.Pls let me know if you have any further questions. Thank you.

靶點詳情

  • 功能：
    Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
  • 基因功能參考文獻：
    1. seven new genetic variations of the IL10 gene were identified. PMID: 24487092
    2. Data indicate that the Leishmania braziliensis antigens plus saponin (LBSap) vaccine induced high levels of IL-12, IL-10, CCL4, CCL5 and CXCL8 expression within 48 hours. PMID: 23911411
    3. serum and tumor levels of IL-8 and IL-10 in dogs bearing benign and malignant mammary tumors, including dogs with inflammatory mammary cancer, for a better understanding of this disease PMID: 23351639
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    IL-10 family
  • 數據庫鏈接：

    KEGG: cfa:403628

    STRING: 9615.ENSCAFP00000016812

    UniGene: Cfa.38



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