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中文名稱:鹿胰島素樣生長因子1(IGF-1)酶聯免疫試劑盒
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貨號:CSB-E12644De
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規格:96T/48T
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價格:¥4200/¥3000
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其他:
產品詳情
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產品描述:鹿胰島素樣生長因子1(IGF-1)酶聯免疫試劑盒(CSB-E12644De)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IGF-1含量。IGF-1(胰島素樣生長因子-1)是一種由IGF1基因編碼的蛋白質,與胰島素具有相似的結構和功能。它主要在肝臟合成,并通過生長激素(GH)的調節促進生長和發育。IGF-1通過激活IGF-1受體,在多個組織和器官中調節細胞增殖、分化和凋亡。其研究機制涉及IGF-1與受體的結合,以及通過多種信號通路(如PI3K/AKT和MEK/ERK)影響細胞功能。試劑盒檢測范圍為1 ng/mL-200 ng/mL,科研人員可將其應用于鹿科動物生長發育研究、營養干預實驗、繁殖生物學分析以及代謝相關疾病模型的機制探索,例如評估不同飼養條件下IGF - 1的動態變化,或探究其在組織再生修復中的作用。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:IGF-1
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Uniprot No.:
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種屬:Deer
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:1 ng/mL-200 ng/mL
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靈敏度:0.5 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Signal Transduction
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of deer IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 98 Range % 92-104 1:2 Average % 95 Range % 89-104 1:4 Average % 99 Range % 87-107 1:8 Average % 96 Range % 90-102 -
回收率:
The recovery of deer IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 89-98 EDTA plasma (n=4) 96 90-102 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average 200 0.175 0.174 0.175 50 0.333 0.316 0.325 16 0.622 0.640 0.631 4 1.040 1.048 1.044 1 1.416 1.389 1.403 0 2.577 2.633 2.605 -
數據處理:
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貨期:3-5 working days
引用文獻
- SWATH-MS Quantitative Proteomic Analysis of Deer Antler from Two Regenerating and Mineralizing Sections M López-Pedrouso,Biology,2021
- Reproductive responses to sexually active buck of does treated with melatonin when body weight/body condition is increasing or decreasing Gallego-Calvo, L.et al, Animal Reproduction Science,2018
- Detection and quantitation of insulin-like growth factor-1 in velvet antler by an enzyme-linked immunosorbent assay /,Consumer Electronics, Communications and Networks (CECNet),2011
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