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Chicken growth hormone,GH ELISA KIT

  • 中文名稱：
    雞生長激素(GH)酶聯免疫試劑盒
  • 貨號：
    CSB-E09866Ch
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產品詳情

  • 產品描述：
    雞生長激素(GH)酶聯免疫試劑盒（CSB-E09866Ch）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GH含量。GH即生長激素，在人體生長發育、代謝等方面扮演關鍵角色。其研究機制主要聚焦于通過與生長激素受體結合，激活相關信號通路，調節細胞增殖、分化和代謝等過程。目前圍繞GH在生長發育異常、代謝疾病等領域的研究不斷深入。試劑盒檢測范圍為625 pg/mL-10000 pg/mL，探究家禽生長發育調控機制、評估營養干預對激素分泌的影響、建立疾病相關代謝模型、農業科研領域研究家禽生理調控、品種選育及飼養管理優化本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    GH ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
  • 縮寫：
    GH
  • Uniprot No.：
  • 種屬：
    Gallus gallus (Chicken)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    625 pg/mL-10000 pg/mL
  • 靈敏度：
    625 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Developmental Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 86-98  
    1:2 Average % 99  
    Range % 92-105  
    1:4 Average % 85  
    Range % 80-89  
    1:8 Average % 90  
    Range % 83-98  
  • 回收率：
    The recovery of chicken GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 88-96  
    EDTA plasma (n=4) 94 91-99  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    10000 0.154 0.145 0.150    
    5000 0.261 0.244 0.253    
    2500 0.538 0.534 0.536    
    1250 1.113 1.139 1.126    
    625 2.356 2.278 2.317    
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Growth hormone plays an important role in growth control.
  • 基因功能參考文獻：
    1. This is the first study to demonstrate that single nucleotide polymorphisms in GHSR can increase appetite, growth traits, expression and level of GHRL, suggesting a hunger signal role for endogenous GHRL. PMID: 27591070
    2. There is a negative glucocorticoid response-like element located in the +591/+609 region within the first intron of chicken growth hormone (cGH), which is essential for the down-regulation of cGH expression. PMID: 27834851
    3. It is shown that in all examined chicken populations the growth hormone gene is polymorphic. PMID: 26841491
    4. The molecular mechanisms underlying glucocorticoid recruitment of somatotrophs during embryogenesis were studied; these provide insight into glucocorticoid-induced developmental changes in other tissues and cell types. PMID: 25560830
    5. T185G growth hormone polymorphism was significantly associated with the egg production traits in chickens. PMID: 25062422
    6. GHRH stimulates testicular GH secretion in an autocrine or paracrine manner. PMID: 24508498
    7. The results indicate that high frequency of I, C and A alleles corresponds to the egg-laying chicken lines. PMID: 23285753
    8. A 1727-bp fragment of 5'-flanking region of chicken growth hormone (cGH) gene has been cloned and sequenced. PMID: 15229358
    9. small chicken GH expression and translation occurs in ocular tissues of chick embryos, in which its localization in the neural retina and the optic nerve head is distinct from that of the full-length protein PMID: 17008400
    10. The identification of 3 restriction enzyme polymorphic sites were detected in the growth hormone gene is reported. PMID: 17495076
    11. Results showed that there was no significant difference between dwarf and normal chickens in GH expression. PMID: 17681929
    12. results demonstrate presence of growth hormone and its receptors in the ovarian stroma with cortical follicles & in the wall of small and large follicles of the ovary during sexual maturation PMID: 18335240
    13. Positive somatotroph responses to corticosterone (CORT) are lost during chicken embryonic development and that glucocorticoid receptors are the primary regulator of CORT-induced growth hormone secretion. PMID: 19157766

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 數據庫鏈接：


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