Chicken adrenocorticotropic hormone (ACTH) ELISA kit

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Chicken adrenocorticotropic hormone (ACTH) ELISA kit

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中文名稱：

雞促腎上腺皮質(zhì)激素(ACTH)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E14373C
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

雞促腎上腺皮質(zhì)激素(ACTH)酶聯(lián)免疫試劑盒（CSB-E14373C）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ACTH含量。促腎上腺皮質(zhì)激素（ACTH）是一種由腦垂體前葉分泌的多肽激素，主要作用是刺激腎上腺皮質(zhì)分泌皮質(zhì)醇，參與調(diào)節(jié)生物體的應(yīng)激反應(yīng)和晝夜節(jié)律。其分泌受下丘腦促腎上腺皮質(zhì)激素釋放激素（CRH）調(diào)控，并通過負(fù)反饋機(jī)制維持激素水平的平衡。研究ACTH有助于理解內(nèi)分泌系統(tǒng)的調(diào)節(jié)機(jī)制和疾病診斷。試劑盒檢測(cè)范圍為0.25 pg/mL-50 pg/mL，適用于家禽養(yǎng)殖業(yè)中應(yīng)激生理研究、實(shí)驗(yàn)室條件下激素分泌調(diào)控機(jī)制分析，以及疾病模型中腎上腺 - 垂體軸功能評(píng)估等科研場(chǎng)景。可為禽類健康管理、環(huán)境適應(yīng)研究及藥理學(xué)實(shí)驗(yàn)中 ACTH 水平監(jiān)測(cè)提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

ACTH
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.25 pg/mL-50 pg/mL
靈敏度：

0.125 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-102
1:2	Average %	91
	Range %	97-95
1:4	Average %	89
	Range %	84-93
1:8	Average %	98
	Range %	92-104

回收率：

The recovery of chicken ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	97	91-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
50	0.176	0.174	0.175
12.5	0.321	0.316	0.319
4	0.534	0.514	0.524
1	0.888	0.857	0.873
0.25	1.432	1.324	1.378
0	1.986	1.905	1.946

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Effect of short-and long-term feed restriction on ghrelin concentrations in turkeys F R Vizcarra.et al,Poultry Science,2018
Concentrations of the adrenocorticotropic hormone, corticosterone and sex steroid hormones and the expression of the androgen receptor in the pituitary and adrenal glands of male turkeys (Meleagris gallopavo) during growth and development Kiezun J.et al,Gen Comp Endocrinol.,2015
Concentrations of the adrenocorticotropic hormone, corticosterone and sex steroid hormones and the expression of the androgen receptor in the pituitary and adrenal ? Kiezun J et al,Gen Comp Endocrinol,2015

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.