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  4. Chicken Interleukin 6,IL-6 ELISA Kit

Chicken Interleukin 6,IL-6 ELISA Kit

  • 中文名稱：
    雞白介素6(IL-6)酶聯免疫試劑盒
  • 貨號：
    CSB-E08549Ch
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：
    雞白介素6(IL-6)酶聯免疫試劑盒（CSB-E08549Ch）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL6含量。IL6是一種多功能細胞因子，由多種細胞產生，參與免疫、炎癥和代謝等生理過程。其研究機制涉及與IL-6R結合，激活JAK/STAT3等信號通路，調節細胞生長、分化和存活。IL6在多種疾病如炎癥、癌癥和自身免疫性疾病中發揮重要作用。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，其動態監測對家禽免疫機制研究、疫苗效力評估及感染性疾病模型構建具有重要意義。適用于科研實驗中定量分析雞類樣本的IL - 6表達水平，可為家禽免疫學基礎研究、病原體感染機制探索及抗炎藥物開發提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    IL6 ELISA Kit; Interleukin-6 ELISA Kit; ChIL-6 ELISA Kit; IL-6 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Gallus gallus (Chicken)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 85-97  
    1:2 Average % 92  
    Range % 85-99  
    1:4 Average % 95  
    Range % 89-100  
    1:8 Average % 92  
    Range % 85-100  
  • 回收率：
    The recovery of chicken IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 91-105  
    EDTA plasma (n=4) 96 88-99  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.487 2.570 2.529 2.454  
    500 2.204 2.230 2.217 2.142  
    250 1.800 1.764 1.782 1.707  
    125 1.390 1.394 1.392 1.317  
    62.5 0.917 0.950 0.934 0.859  
    31.2 0.584 0.568 0.576 0.501  
    15.6 0.348 0.362 0.355 0.280  
    0 0.074 0.075 0.075 　  
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 最新研究進展：
    IL-6是一種多功能細胞因子，可以影響許多不同類型的細胞和器官。最新研究表明，IL-6參與了許多疾病的發病機制，如自身免疫性疾病、心血管疾病、腫瘤等。此外，IL-6在新冠疫情中也扮演了重要角色，成為疫苗研究和治療的關鍵目標之一。針對IL-6的治療方法，如抗IL-6受體抗體和抑制IL-6表達的藥物等，在臨床治療中已經取得了一些成功。
  • 功能：
    Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.
  • 基因功能參考文獻：
    1. Avian leukosis virus subgroup J induces VEGF expression via NF-kappaB/PI3K-dependent IL-6 production to promoter tumorigenesis. PMID: 27852059
    2. The effects of daily corticosterone injections on acute phase proteins (APP), IL-6, and HSP70 are reported; APP and HSP70 may be of value as indicators of stress in poultry. PMID: 25306460
    3. Chicken IL-6 is a heat-shock gene PMID: 24055475
    4. Nampt/visfatin/PBEF affects expression of myogenic regulatory factors and is regulated by interleukin-6 in chicken skeletal muscle cells PMID: 21545843
    5. IL-6 is involved in the progression of chicken arthritis induced by Staph. aureus, and it contributes to disease incidence and mortality PMID: 17495100

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    IL-6 superfamily
  • 數據庫鏈接：

    KEGG: gga:395337

    UniGene: Gga.2769



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