Chicken Interleukin 2,IL-2 ELISA Kit
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中文名稱:雞白介素2(IL-2)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E06755Ch
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規(guī)格:96T/48T
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價(jià)格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:雞白介素2(IL-2)酶聯(lián)免疫試劑盒(CSB-E06755Ch)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的IL-2含量。IL-2是重要的免疫調(diào)節(jié)細(xì)胞因子,在免疫系統(tǒng)中作用關(guān)鍵。其背景上,它能參與T細(xì)胞、B細(xì)胞等免疫細(xì)胞的增殖分化。研究機(jī)制方面,主要通過(guò)與細(xì)胞表面IL-2受體結(jié)合,激活下游信號(hào)通路,從而調(diào)節(jié)免疫細(xì)胞功能,在腫瘤免疫、自身免疫病治療研究中受關(guān)注。試劑盒檢測(cè)范圍為0.18 pg/mL-12 pg/mL,科研人員可將其應(yīng)用于家禽免疫機(jī)制研究、疫苗效力評(píng)估或疾病模型中細(xì)胞免疫狀態(tài)的動(dòng)態(tài)監(jiān)測(cè),特別適用于分析感染性疾病、炎癥反應(yīng)及免疫調(diào)節(jié)實(shí)驗(yàn)中IL - 2的表達(dá)水平變化。為實(shí)驗(yàn)室開(kāi)展禽類免疫學(xué)研究提供了標(biāo)準(zhǔn)化檢測(cè)工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫:IL-2
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Uniprot No.:
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種屬:Gallus gallus (Chicken)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:0.18 pg/mL-12 pg/mL
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靈敏度:0.04 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Immunology
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 89 Range % 85-96 1:2 Average % 90 Range % 85-97 1:4 Average % 99 Range % 94-106 1:8 Average % 97 Range % 92-104 -
回收率:
The recovery of chicken IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 100 92-109 EDTA plasma (n=4) 96 90-104 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 12 2.151 2.198 2.175 2.072 6 1.835 1.869 1.852 1.749 3 1.457 1.487 1.472 1.369 1.5 1.098 1.051 1.075 0.972 0.75 0.698 0.682 0.690 0.587 0.38 0.449 0.460 0.455 0.352 0.18 0.236 0.222 0.229 0.126 0 0.102 0.104 0.103 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Impact of multistrain probiotics on growth performance, immune response, and gut morphometry in broiler chicken Gallus gallus domesticus S Younas, DA Bukhari, Z Bibi, A Ullah, A Rehman,Poultry Science,2025
- Influence of Graded Levels of l-Theanine Dietary Supplementation on Growth Performance, Carcass Traits, Meat Quality, Organs Histomorphometry, Blood Chemistry and Immune Response of Broiler Chickens Saeed M,International journal of molecular sciences,2023
- Effects of essential oil/palygorskite composite on performance, egg quality, plasma biochemistry, oxidation status, immune response and intestinal morphology of laying hens H.Cheng,Poultry Science,2021
- Effects of different levels of Hermetia illucens larvae meal on performance, egg quality, yolk fatty acid composition and oxidative status of laying hens Xin Liu,Italian Journal of Animal Science,2021
- Enhanced cytokine expression and upregulation of inflammatory signaling pathways in broiler chickens affected by wooden breast myopathy T Xing,J. Sci. Food Agric,2020
- A Novel Adjuvant “Sublancin” Enhances Immune Response in Specific Pathogen-Free Broiler Chickens Inoculated with Newcastle Disease Vaccine Liu Y, et al,Journal of Immunology Research,2019
- Effect of the melatonin nuclear receptor RORα on monochromatic light-induced T-lymphocyte proliferation in chicken thymus Xiong J, et al,Immunology Letters,2019
- The effects and combinational effects of Bacillus subtilis and montmorillonite supplementation on performance, egg quality, oxidation status, and immune response in laying hens J.F.Chen, et al,The Egyptian Journal of Neurology, Psychiatry and Neurosurgery,2019
- Effects of Supplementing Laying Hens with Purified Amino Acid Prepared from Animal Blood N. Joshi, et al,Tropical animal science journal,2019
- Effects of three probiotic Bacillus on growth performance, digestive enzyme activities, antioxidative capacity, serum immunity, and biochemical parameters in broilers Li Gong.et al,animal science journal,2018
- Dietary Supplementation of Purified Amino Acid Derived from Animal Blood on Immune Response and Growth Performance of Broiler Chicken T. G. Wandita.et al,Tropical Animal Science Journal,2018
- The molecular characterization and protective efficacy of microneme 3 of Eimeria mitis in chickens XinmeiHuang.et al,Veterinary Parasitology,2018
- Effects of modified montmorillonite adsorbent on performance, egg quality, serum biochemistry, oxidation status, and immune response of laying hens in late Qu, X. Y.et al,Livestock Science,2018
- The effect of heat stress on intestinal integrity and Salmonella invasion in broiler birds Alhenaky A.et al,J Therm Biol.,2017
- Dietary Supplementation of Phoenix dactylifera Seeds Enhances Performance, Immune Response, and Antioxidant Status in Broilers Ali H. El-Far.et al,Oxidative Medicine and Cellular Longevity,2016
- Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses. Paudel S.et al,Virus Res.,2016
- The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina. Zhang Z.et al,J Eukaryot Microbiol.,2016
- The molecular characterization and immune protection of microneme 2 of Eimeria acervulina ZC Zhang. et al,Veterinary Parasitology,2015
- Immune responses to oral vaccination with Salmonella-delivered avian pathogenic Escherichia coli antigens and protective efficacy against colibacillosis. Lee JH.et al,Can J Vet Res.,2015
- Omega-3 polyunsaturated fatty acids enrichment alters performance and immune response in infectious bursal disease challenged broilers Elham Maroufyan et al,Lipids in Health and Disease,2012
- Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV to Marek's Disease Virus (MDV) Type-1 VP22 protein Hassan Moeini et al,Virology Journal,2011
- Lactobacillus acidophilus as a live vehicle for oral immunization against chicken anemia virus Hassan Moeini et al,Tumor Biology,2010
- Immune protection of Microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens Huang J. et al,Avian Pathol,2015
- Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge Huang J. et al,Parasitol Int,2015
- Consumption of Oxidized Soybean Oil Increased Intestinal Oxidative Stress and Affected Intestinal Immune Variables in Yellow-feathered Broilers Fangfang Liang. et al,Asian Australas. J. Anim. Sci. ,2015
- Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV /,Comparative Immunology, Microbiology and Infectious Diseases,2011
- Bursopentine as a Novel Immunoadjuvant Enhances both Humoral and Cell-Mediated Immune Responses to Inactivated H9N2 Avian Influenza Virus in Chickens /,clinical and vaccine immunology,2011
靶點(diǎn)詳情
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最新研究進(jìn)展:IL-2是一種細(xì)胞因子,可刺激T細(xì)胞和自然殺傷細(xì)胞的增殖和活化。最新研究表明,IL-2在癌癥和免疫治療方面具有潛在的應(yīng)用價(jià)值。例如,IL-2在肝癌和結(jié)直腸癌等方面的臨床應(yīng)用已經(jīng)得到研究。此外,IL-2還被用于免疫治療,如通過(guò)IL-2增強(qiáng)T細(xì)胞治療腫瘤的方法。
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