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  4. Chicken Interleukin 10,IL-10 ELISA Kit

Chicken Interleukin 10,IL-10 ELISA Kit

  • 中文名稱：
    雞白介素10(IL-10)酶聯免疫試劑盒
  • 貨號：
    CSB-E12835C
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：
    雞白介素10(IL-10)酶聯免疫試劑盒（CSB-E12835C）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL10含量。IL10是重要的細胞因子，具有抗炎特性，可抑制促炎細胞因子產生。其在維持機體免疫平衡中有關鍵作用。研究機制上，主要通過與受體結合，激活相關信號通路，抑制炎癥介質釋放，在炎癥性疾病等研究中受廣泛關注。試劑盒檢測范圍為1 pg/mL-200 pg/mL，支持科研人員高效檢測雞感染性疾病模型、疫苗免疫效果評估或免疫調節機制研究中的IL - 10動態變化。適用于家禽免疫學、病理學及生物制劑開發等科研領域本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Gallus gallus (Chicken)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1 pg/mL-200 pg/mL
  • 靈敏度：
    0.5 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 90-101  
    1:2 Average % 97  
    Range % 94-105  
    1:4 Average % 95  
    Range % 89-100  
    1:8 Average % 92  
    Range % 87-96  
  • 回收率：
    The recovery of chicken IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 97 93-101  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average 　  
    200 0.155 0.152 0.154 　  
    50 0.334 0.348 0.341 　  
    16 0.515 0.502 0.509 　  
    4 0.969 0.975 0.972 　  
    1 1.436 1.463 1.450 　  
    0 2.658 2.667 2.663 　  
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 最新研究進展：
    IL-10是一種抗炎因子，對調節炎癥反應和維持免疫平衡具有重要作用。最新研究發現，IL-10參與許多自身免疫性疾病的發病機制，并且IL-10的水平可能作為這些疾病的生物標記物。此外，IL-10在腫瘤微環境中也發揮作用，可以通過抑制炎癥反應和免疫應答來促進腫瘤生長。
  • 功能：
    Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    IL-10 family
  • 組織特異性：
    Expressed predominantly in bursa of Fabricius and cecal tonsils with low levels in thymus, liver and lung.
  • 數據庫鏈接：


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