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中文名稱:雞β干擾素(IFN-β/IFNB)酶聯免疫試劑盒
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貨號:CSB-E11237Ch
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規格:96T/48T
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價格:¥4200/¥3000
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其他:
產品詳情
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產品描述:雞β干擾素(IFN-β/IFNB)酶聯免疫試劑盒(CSB-E11237Ch)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IFN-β/IFNB含量。IFN-β/IFNB即β-干擾素,是機體受病毒感染后產生的一類細胞因子。它能與細胞表面受體結合,激活下游信號通路,誘導抗病毒蛋白產生,抑制病毒復制和調節免疫反應,在抗病毒、抗腫瘤及免疫相關疾病研究中受關注。試劑盒檢測范圍為2.5 pg/mL-200 pg/mL,適用于科研場景中禽類免疫機制研究、疫苗效力評價或抗病毒藥物開發等方向;可應用于評估病毒感染模型、免疫刺激實驗或比較不同組織微環境中IFN-β的動態表達水平本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:IFNB ELISA Kit; IFN2 ELISA Kit; Interferon type B ELISA Kit; IFN-beta ELISA Kit
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縮寫:IFN-β/IFNB
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Uniprot No.:
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種屬:Gallus gallus (Chicken)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:2.5 pg/mL-200 pg/mL
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靈敏度:3.125 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Immunology
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 99 Range % 95-103 1:2 Average % 94 Range % 88-98 1:4 Average % 102 Range % 98-106 1:8 Average % 89 Range % 84-96 -
回收率:
The recovery of chicken IFN-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 88-94 EDTA plasma (n=4) 88 82-96 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 200 0.342 0.341 0.342 75 0.494 0.486 0.490 37.5 0.652 0.664 0.658 12.5 1.067 1.076 1.072 2.5 1.478 1.575 1.527 0 1.854 1.955 1.905 -
數據處理:
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貨期:3-5 working days
引用文獻
- NGPV VP1 targets IRF7 protein to block the type I interferon upstream signaling pathway Y Yang,Veterinary research,2024
- Differences in expression of genes in the MyD88 and TRIF signalling pathways and methylation of TLR4 and TRIF in Tibetan chickens and DaHeng S03 chickens infected with Salmonella enterica serovar Enteritidis Xiaocheng Li.et al,Veterinary Immunology and Immunopathology,2017
相關產品
靶點詳情
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功能:Has antiviral activities.
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基因功能參考文獻:
- Fpv012 inhibits induction of IFNB. PMID: 23427153
- RNA interference-mediated knockdown of ChMda5 showed that ChMda5 plays an important role in the IFN response of chicken cells to dsRNA PMID: 21444763
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亞細胞定位:Secreted.
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蛋白家族:Alpha/beta interferon family
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數據庫鏈接:
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