Chicken Glutathione Peroxidase 8 (GPX-8) ELISA Kit
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中文名稱:雞谷胱甘肽過氧化物酶8(GPX-8)酶聯免疫試劑盒
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貨號:CSB-E14942C
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規格:96T/48T
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價格:¥4200/¥3000
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其他:
產品詳情
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產品描述:雞谷胱甘肽過氧化物酶8(GPX-8)酶聯免疫試劑盒(CSB-E14942C)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的GPX-8含量。雞谷胱甘肽過氧化物酶8(GPX-8)是一種位于內質網的非特異性抗氧化酶,參與清除活性氧,保護細胞膜免受氧化損傷。其基因突變與腫瘤發生密切相關,在多種腫瘤組織中異常表達,影響腫瘤免疫微環境和侵襲轉移。試劑盒檢測范圍為31.25 mU/mL-2000 mU/mL,該產品可廣泛應用于家禽氧化應激機制研究、營養代謝評估、毒理學實驗及疾病模型建立等科研領域,例如評估飼料抗氧化劑效果、監測環境毒素暴露對家禽抗氧化系統的影響,或探究傳染性疾病進程中GPX - 8的動態變化規律,為禽類健康管理和基礎醫學研究提供可靠的技術支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:GPX-8
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Uniprot No.:
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種屬:Gallus gallus (Chicken)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:31.25 mU/mL-2000 mU/mL
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靈敏度:7.8 mU/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Metabolism
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of chicken GPX-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 98 Range % 94-103 1:200 Average % 86 Range % 83-90 1:400 Average % 104 Range % 100-108 1:800 Average % 89 Range % 85-93 -
回收率:
The recovery of chicken GPX-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 107 103-110 EDTA plasma (n=4) 84 80-88 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. mU/ml OD1 OD2 Average 2000 0.111 0.115 0.113 1000 0.208 0.210 0.209 500 0.373 0.390 0.382 250 0.642 0.638 0.640 125 0.956 0.961 0.959 62.5 1.146 1.200 1.173 31.25 1.417 1.431 1.424 0 1.634 1.645 1.640 -
數據處理:
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貨期:3-5 working days
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