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Chicken Ghrelin ELISA Kit

  • 中文名稱:
    雞饑餓激素酶聯免疫試劑盒
  • 貨號:
    CSB-E14230C
  • 規格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 其他:

產品詳情

  • 產品描述:
    雞饑餓激素酶聯免疫試劑盒(CSB-E14230C)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的Ghrelin含量。Ghrelin是一種重要靶點。它是胃內產生的肽,能調節食欲和能量穩態。研究機制方面,Ghrelin與受體結合后,作用于神經內分泌系統,影響下丘腦信號通路,調節攝食、生長激素釋放等,在肥胖、代謝疾病等研究中受關注。試劑盒檢測范圍為25 pg/mL-400 pg/mL,適用于家禽生理學研究、營養代謝實驗、生長調控機制探索等科研領域,如評估飼料成分對Ghrelin分泌的影響或分析激素水平與生長性能的關聯性,為禽類生物學研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    Ghrelin
  • 種屬:
    Gallus gallus (Chicken)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    25 pg/mL-400 pg/mL
  • 靈敏度:
    25 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken ghrelin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 87-96  
    1:2 Average % 90  
    Range % 86-95  
    1:4 Average % 95  
    Range % 90-100  
    1:8 Average % 97  
    Range % 92-103  
  • 回收率:
    The recovery of chicken ghrelin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-98  
    EDTA plasma (n=4) 93 88-98  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    400 0.248 0.244 0.246    
    200 0.506 0.500 0.503    
    100 0.863 0.856 0.860    
    50 1.687 1.702 1.695    
    25 2.515 2.423 2.469    
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價



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