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Canine trypsin ELISA Kit

  • 中文名稱:
    犬胰蛋白酶(trypsin)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E12804c
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    犬胰蛋白酶(trypsin)酶聯(lián)免疫試劑盒(CSB-E12804c)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的trypsin含量。Trypsin即胰蛋白酶,是一種絲氨酸蛋白酶。它在人體消化過(guò)程中起關(guān)鍵作用,能水解蛋白質(zhì)。在研究方面,胰蛋白酶可作為藥物靶點(diǎn),用于開(kāi)發(fā)治療胰腺炎等疾病的藥物,同時(shí)也用于一些生物工藝,如細(xì)胞培養(yǎng)實(shí)驗(yàn)中解離細(xì)胞。試劑盒檢測(cè)范圍為39 ng/mL-2500 ng/mL,該產(chǎn)品適用于科研領(lǐng)域中對(duì)犬類胰腺生理機(jī)制、炎癥反應(yīng)或營(yíng)養(yǎng)代謝相關(guān)課題的探索,例如通過(guò)檢測(cè)不同實(shí)驗(yàn)條件下胰蛋白酶表達(dá)動(dòng)態(tài),解析其與疾病模型的關(guān)聯(lián)性或評(píng)估藥物干預(yù)效果。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 縮寫:
    trypsin
  • 種屬:
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    39 ng/mL-2500 ng/mL
  • 靈敏度:
    9.75 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of canine trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %97
    Range %85-106
    1:2Average %93
    Range %85-105
    1:4Average %95
    Range %82-102
    1:8Average %95
    Range %84-110
  • 回收率:
    The recovery of canine trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9687-107
    EDTA plasma (n=4)9786-105
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    25002.263 2.319 2.291 2.162
    12501.861 1.954 1.908 1.779
    6251.365 1.372 1.369 1.240
    3120.874 0.898 0.886 0.757
    1560.592 0.583 0.588 0.459
    780.307 0.298 0.303 0.174
    390.232 0.246 0.239 0.110
    00.127 0.131 0.129  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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