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  4. Canine thymus activation regulated chemokine(TARC/CCL17)ELISA Kit

Canine thymus activation regulated chemokine(TARC/CCL17)ELISA Kit

  • 中文名稱：
    犬胸腺活化調(diào)節(jié)趨化因子(TARC/CCL17)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E17792c
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬胸腺活化調(diào)節(jié)趨化因子(TARC/CCL17)酶聯(lián)免疫試劑盒（CSB-E17792c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TARC/CCL17含量。TARC/CCL17是重要的靶點(diǎn)。它是一種趨化因子，在免疫系統(tǒng)中發(fā)揮關(guān)鍵作用。其異常表達(dá)與多種疾病如特應(yīng)性皮炎、哮喘等相關(guān)。研究機(jī)制主要聚焦于其與受體結(jié)合，介導(dǎo)免疫細(xì)胞遷移、活化，進(jìn)而影響疾病的發(fā)生發(fā)展，為相關(guān)疾病治療提供潛在方向。試劑盒檢測(cè)范圍為23.5 pg/mL-1500 pg/mL，該產(chǎn)品可為犬類過敏性皮炎模型研究、呼吸道炎癥機(jī)制探索、免疫調(diào)節(jié)藥物開發(fā)等科研方向提供可靠數(shù)據(jù)支持，尤其適用于動(dòng)態(tài)監(jiān)測(cè)疾病進(jìn)程中趨化因子表達(dá)變化或評(píng)估實(shí)驗(yàn)性治療干預(yù)效果，為深入解析犬類免疫調(diào)控網(wǎng)絡(luò)提供技術(shù)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    CCL17 ELISA Kit; TARCC-C motif chemokine 17 ELISA Kit; CC chemokine TARC ELISA Kit; Small-inducible cytokine A17 ELISA Kit; Thymus and activation-regulated chemokine ELISA Kit
  • 縮寫：
    TARC/CCL17
  • Uniprot No.：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    23.5 pg/mL-1500 pg/mL
  • 靈敏度：
    5.86 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine TARC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %87
    Range %84-90
    1:2Average %102
    Range %97-107
    1:4Average %91
    Range %85-97
    1:8Average %95
    Range %91-99
  • 回收率：
    The recovery of canine TARC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-99
    EDTA plasma (n=4)10097-103
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15001.908 2.005 1.957 1.801
    7501.179 1.205 1.192 1.036
    3750.734 0.738 0.736 0.580
    187.50.415 0.436 0.426 0.270
    940.311 0.328 0.320 0.164
    470.231 0.245 0.238 0.082
    23.50.198 0.189 0.194 0.038
    00.155 0.157 0.156
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Chemotactic factor for t lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4 and CCR8.
  • 基因功能參考文獻(xiàn)：
    1. The present results suggest that TNF-alpha-induced CCL17 mRNA transcription in canine keratinocytes is positively regulated by p38 but negatively controlled by ERK. PMID: 20837364
    2. This study suggests that interferon-gamma may suppress granulocyte-macrophage colony stimulating factor production from canine keratinocytes, although further studies are required to confirm this. PMID: 20860556
    3. Inflammatory cytokines are important inducing factors for the production of CCL17 in the lesional skin of dogs with atopic dermatitis. atopic dermatitis. PMID: 20022349
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 組織特異性：
    Expressed in thymus, spleen, lymph node, lung and heart.
  • 數(shù)據(jù)庫(kù)鏈接：


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