Canine acetylcholine receptor antibody,AChRab ELISA Kit

產品詳情

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說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

犬乙酰膽堿受體抗體(AChRab)酶聯免疫試劑盒（CSB-E08375c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的AChR Ab含量。AChR Ab即乙酰膽堿受體抗體，它與重癥肌無力等疾病密切相關。其背景在于機體免疫系統異常產生該抗體。研究機制主要是它會與神經肌肉接頭處的乙酰膽堿受體結合，干擾神經沖動傳導，影響肌肉正常收縮，是相關疾病發病的重要因素。試劑盒檢測范圍為3.75 ng/mL-240 ng/mL，適用于基礎科研中探索AChRab在神經功能障礙模型中的表達變化，或用于藥物開發過程中評估實驗性治療對抗體水平的調控作用，同時也可作為犬類自身免疫疾病研究中的生物標志物檢測方案，為動物模型的建立及病理機制解析提供數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

AChR Ab
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma
檢測范圍：

3.75 ng/mL-240 ng/mL
靈敏度：

0.94 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine AChRab in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-102
1:2	Average %	95
	Range %	84-100
1:4	Average %	96
	Range %	90-103
1:8	Average %	94
	Range %	82-101

回收率：

The recovery of canine AChRab spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	92	87-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.