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中文名稱:犬孕激素/孕酮(PROG)酶聯免疫試劑盒
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貨號:CSB-E07285c
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規格:96T/48T
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價格:¥4200/¥3000
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其他:
產品詳情
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產品描述:犬孕激素/孕酮(PROG)酶聯免疫試劑盒(CSB-E07285c)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿.樣本中的PROG含量。試劑盒檢測范圍為0.15 ng/ml-70 ng/ml,靈敏度為0.2 ng/ml。適用于動物繁殖生理研究、生殖內分泌機制探索、藥物對卵巢功能影響評估等場景,尤其可為犬類人工授精時機判斷、妊娠監測及生殖疾病模型構建提供可靠數據支持本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:PROG
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種屬:Canis lupus familiaris (Dog) (Canis familiaris)
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樣本類型:serum, plasma, tissue homogenates.
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檢測范圍:0.15 ng/ml-70 ng/ml.
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靈敏度:0.2 ng/ml.
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Signal Transduction
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of canine PROG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
96
Range %
92-104
1:2
Average %
94
Range %
91-99
1:4
Average %
102
Range %
98-105
1:8
Average %
89
Range %
82-94
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回收率:
The recovery of canine PROG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
93
90-97
EDTA plasma (n=4)
101
98-105
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標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
0
2.284
2.275
2.280
0.15
1.429
1.589
1.509
1
0.843
0.874
0.859
5
0.566
0.585
0.576
20
0.362
0.353
0.358
70
0.201
0.202
0.202
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數據處理:
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貨期:3-5 working days
引用文獻
- The evaluation of the oxidative stress index of reproductive tissues and serum thiol/disulphide homeostasis during estrous cycle in bitches Acar D B,Ankara ?niversitesi Veteriner Fak眉ltesi Dergisi,2019
- Anti-Müllerian hormone as a diagnostic tool for ovarian remnant syndrome in bitches. Turna Yilmaz Ö. et al,Vet Res Commun,2015
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