Canine Matrix metalloproteinase 1,MMP-1 ELISA kit
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中文名稱：犬基質(zhì)金屬蛋白酶1(MMP-1)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E14057c
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：犬基質(zhì)金屬蛋白酶1(MMP-1)酶聯(lián)免疫試劑盒（CSB-E14057c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的MMP-1含量。MMP-1，又稱間質(zhì)膠原酶和成纖維細(xì)胞膠原酶，是基質(zhì)金屬蛋白酶家族的一員，主要參與細(xì)胞外基質(zhì)的降解。其在正常生理過程中如胚胎發(fā)育和組織重塑中起作用，同時(shí)在疾病過程中如癌癥和骨關(guān)節(jié)炎中，MMP-1的表達(dá)和活性增加，導(dǎo)致細(xì)胞外基質(zhì)破壞，促進(jìn)疾病進(jìn)展。試劑盒檢測(cè)范圍為0.18 ng/mL-12 ng/mL，適用于科研場(chǎng)景中探究犬類疾病模型（如關(guān)節(jié)炎、皮膚損傷修復(fù)或腫瘤微環(huán)境）中MMP-1的動(dòng)態(tài)變化。可為分子機(jī)制研究、藥物開發(fā)或生物標(biāo)志物篩選提供定量數(shù)據(jù)支持，滿足科研工作者對(duì)犬類MMP-1活性監(jiān)測(cè)的需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫：MMP-1
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種屬：Canis lupus familiaris (Dog) (Canis familiaris)
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：0.18 ng/mL-12 ng/mL
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靈敏度：0.04 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Cancer
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 85-102 1:2 Average % 89 Range % 85-99 1:4 Average % 94 Range % 85-97 1:8 Average % 90 Range % 83-98 -
回收率：
The recovery of canine MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 82-98 EDTA plasma (n=4) 97 89-100 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 12 2.309 2.326 2.318 2.114 6 1.754 1.699 1.727 1.523 3 1.301 1.338 1.320 1.116 1.5 0.931 0.920 0.926 0.722 0.75 0.573 0.585 0.579 0.375 0.37 0.412 0.407 0.410 0.206 0.18 0.299 0.303 0.301 0.097 0 0.201 0.206 0.204 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Inflammatory pattern of the infrapatellar fat pad in dogs with canine cruciate ligament disease Manuel R. Schmidli.et al,BMC VET RES,2018
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