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  4. Canine Cardiac Troponin Ⅰ(cTn-Ⅰ) ELISA kit

Canine Cardiac Troponin Ⅰ(cTn-Ⅰ) ELISA kit

  • 中文名稱：
    犬心肌肌鈣蛋白Ⅰ(cTn-Ⅰ)酶聯免疫試劑盒 Kit
  • 貨號：
    CSB-E08772c
  • 規格：
    96T/48T
  • 價格：
    ￥4800/￥3000
  • 其他：

產品詳情

  • 產品描述：
    犬心肌肌鈣蛋白Ⅰ(cTn-Ⅰ)酶聯免疫試劑盒 Kit（CSB-E08772c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的cTn-I含量。cTn - I是心肌肌鈣蛋白I，是心肌損傷的特異性標志物。其背景在于在心肌受損時釋放入血，可反映心肌損傷情況。研究機制主要聚焦在檢測其在血液中的含量變化，用于早期診斷急性心肌梗死等心肌損傷相關疾病。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，本產品適用于科研領域，如犬類心肌病機制探索、心臟毒性藥物評價或運動醫學研究中心肌微損傷監測，為獸醫基礎研究、實驗動物模型構建及比較醫學分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    TNNI3 ELISA Kit; Troponin I ELISA Kit; cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit
  • 縮寫：
    cTn-
  • Uniprot No.：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    23.5 pg/mL-1500 pg/mL
  • 靈敏度：
    5.8 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %90-100
    1:2Average %89
    Range %85-99
    1:4Average %98
    Range %91-104
    1:8Average %96
    Range %91-100
  • 回收率：
    The recovery of canine cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9792-108
    EDTA plasma (n=4)9590-101
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15002.245 2.250 2.248 2.071
    7501.716 1.704 1.710 1.533
    3751.144 1.161 1.153 0.976
    187.50.790 0.775 0.783 0.606
    940.459 0.474 0.467 0.290
    470.305 0.289 0.297 0.120
    23.50.215 0.211 0.213 0.036
    00.176 0.177 0.177 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 基因功能參考文獻：
    1. No statistical differences were found between CRP and cTnI levels, either before or after surgical correction of brachycephaly in dogs. PMID: 25673910
    2. The objective of this study was to evaluate the correlation between anatomical components, clinical signs and several biomarkers, used to determine systemic inflammation and myocardial damage in dogs with brachycephalic upper airway obstructive syndrome. PMID: 22937913
    3. The use of NT-pro-BNP and troponin I levels as prognostic indicators for canine heart failure is reported. PMID: 22439324
    4. [NT-proBNP] concentration is significantly higher in dogs with pre-capillary pulmonary hypertension (PH) when compared to dogs with respiratory disease without PH; [NT-proANP] and [cTnI] were not elevated in dogs with pre-capillary PH. PMID: 21835711
    5. Moderate exercise does not present a confounding variable in the interpretation of cardiac tropinin I and C-reactive protein concentrations in normal dogs. PMID: 20840303
    6. Serum troponin I is high in dogs with either precapillary and postcapillary pulmonary hypertension (PH); myocardial damage in dogs with postcapillary PH is likely the consequence of increased mitral valve disease severity. PMID: 20002552
    7. Analysis of troponin I concentration has potential to increase knowledge of overall cardiac remodeling in dogs with mitral valve disease; the effect of age on troponin I levels should be considered in these patients. PMID: 20002554
    8. Circulating cTnI and pro-matrix metallopeptidase 9 increase in proportion to the severity of acute pulmonary thromboembolism PMID: 18047836
    9. The incidence of myocardial injury due to ehrlichiosis, as demonstrated by elevated cardiac troponin I blood levels, is reported. PMID: 18638021

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  • 蛋白家族：
    Troponin I family
  • 數據庫鏈接：

    KEGG: cfa:403566

    STRING: 9615.ENSCAFP00000003805

    UniGene: Cfa.83



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