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Bovine pulmonary surfactant-associated protein B,SP-B ELISA Kit

  • 中文名稱:
    牛肺表面活性物質相關蛋白B(SP-B)酶聯免疫試劑盒
  • 貨號:
    CSB-E13834B
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    牛肺表面活性物質相關蛋白B(SP-B)酶聯免疫試劑盒(CSB-E13834B)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的SFTPB含量。試劑盒檢測范圍為9.37 ng/mL-600 ng/mL,適用于研究SP - B在肺部生理功能、病理機制(如急性肺損傷或發育異常)中的表達調控,也可用于評估動物模型中藥物干預或基因編輯對SP - B水平的影響。其兼容多種樣本類型的特點,為科研人員在基礎研究和轉化醫學領域探索呼吸系統相關分子機制提供了高效、可靠的分析工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    SFTPB ELISA Kit; SFTP3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; 6 kDa protein ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
  • 縮寫:
    SFTPB
  • Uniprot No.:
  • 種屬:
    Bos taurus (Bovine)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    9.37 ng/mL-600 ng/mL
  • 靈敏度:
    2.34 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %92-103
    1:2Average %91
    Range %85-97
    1:4Average %90
    Range %88-94
    1:8Average %100
    Range %96-105
  • 回收率:
    The recovery of bovine SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9087-93
    EDTA plasma (n=4)106103-108
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    6002.238 2.412 2.325 2.191
    3001.603 1.620 1.612 1.478
    1501.054 1.102 1.078 0.944
    750.766 0.771 0.769 0.635
    37.50.534 0.580 0.557 0.423
    18.750.322 0.329 0.326 0.192
    9.370.234 0.219 0.227 0.093
    00.132 0.135 0.134
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
  • 基因功能參考文獻:
    1. Measurements of the depth of SP-B(CTERM) indicated the peptide center positions ~8A more deeply than the phosphate headgroups, a topology that may allow the peptide to promote functional lipid structures without causing micellization upon compression. PMID: 22252270
    2. results suggest the hydrophobic surfactant protein, SP-B, protects alveolar surfactant phospholiopids from hydrolysis mediated by multiple sPLA(2) in both vesicles (alveolar subphase) and monomolecular films (air-liquid interface) PMID: 22037357
    3. The hydrophobic surfactant proteins SP-B and SP-C induce formation of bicontinuous inverse cubic phases. PMID: 20409474
    4. Reactive oxygen species inactivation of lipid extract surfactant B (SP-B) biophysical activity appears to involve modification of a maximum of three amino acids: Trp9, Met29, and Met65. PMID: 17425286
  • 亞細胞定位:
    Secreted, extracellular space, surface film.
  • 數據庫鏈接:


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