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Bovine fibroblast growth factor 21 (FGF21) ELISA kit

文獻引用 (3) 說明書下載

產品詳情

產品描述：

牛成纖維細胞生長因子21(FGF21)酶聯免疫試劑盒（CSB-EL008627BO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FGF21含量。FGF21，即成纖維細胞生長因子21，是一種具有多效性的代謝調節劑，對心血管系統具有保護作用，主要通過與FGFR受體的相互作用發揮作用，參與代謝調節，與肥胖和糖尿病等代謝綜合征相關。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于獸醫科學研究、動物生理學實驗及畜牧領域代謝相關分子機制探索，例如在飼料效率優化、代謝綜合征模型構建等研究中定量分析FGF21的表達動態本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

FGF21
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine FGF21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-93
1:2	Average %	101
	Range %	95-105
1:4	Average %	97
	Range %	92-101
1:8	Average %	88
	Range %	83-96

回收率：

The recovery of bovine FGF21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-90
EDTA plasma (n=4)	99	95-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.