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Bovine Vascular endothelial growth factor A(VEGFA) ELISA kit

  • 中文名稱:
    牛血管內(nèi)皮生長因子A(VEGFA)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-EL025833BO
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    牛血管內(nèi)皮生長因子A(VEGFA)酶聯(lián)免疫試劑盒(CSB-EL025833BO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的VEGFA含量。VEGFA即血管內(nèi)皮生長因子A,是一種促進血管生成的重要因子。其背景在于在腫瘤、眼科疾病等病理狀態(tài)下表達常異常升高。研究機制主要是通過與受體結(jié)合,激活下游信號通路,促進血管內(nèi)皮細胞的增殖、遷移,形成新生血管,為疾病發(fā)展提供營養(yǎng)支持。試劑盒檢測范圍為47 pg/mL-3000 pg/mL,本產(chǎn)品適用于牛VEGFA表達調(diào)控機制研究、心血管疾病模型評估、腫瘤血管生成實驗及生殖生理相關(guān)科研項目,為獸醫(yī)基礎(chǔ)研究、畜牧業(yè)抗病育種及生物制藥領(lǐng)域提供可靠的檢測工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    VEGFA ELISA kit; VEGFVascular endothelial growth factor A ELISA kit; VEGF-A ELISA kit; Vascular permeability factor ELISA kit; VPF ELISA kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Bos taurus (Bovine)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    47 pg/mL-3000 pg/mL
  • 靈敏度:
    11.75 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine VEGFA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 97
    Range % 88102
    1:2 Average % 92
    Range % 87-92
    1:4 Average % 90
    Range % 85-94
    1:8 Average % 92
    Range % 84-96
  • 回收率:
    The recovery of bovine VEGFA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 84-95
    EDTA plasma (n=4) 104 97-111
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    3000 2.147 1.988 2.068 1.940
    1500 1.604 1.576 1.590 1.462
    750 1.105 1.089 1.097 0.969
    375 0.756 0.767 0.762 0.634
    187.5 0.538 0.531 0.535 0.407
    94 0.340 0.332 0.336 0.208
    47 0.228 0.221 0.225 0.097
    0 0.127 0.129 0.128  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

相關(guān)問答

 常見問題解答
Q:

I was wondering if you have a VEGF ELISA kit that I can test for both Human and Bovine samples? This is the Bovine code CSB-EL025833BO.

A:
Thanks for your inquiry!
We suggest you use bovine kit CSB-EL025833BO to test bovine samples and human kit CSB-E11718h to test human samples because these kits are developed against different matrix. The antibodies used in the kits have different resistance to matrix interference.

靶點詳情

  • 功能:
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development.
  • 基因功能參考文獻:
    1. data for the first time demonstrate a calpain/PTP1B/VEGFR2 negative feedback loop in the regulation of VEGF-induced angiogenesis. Modulation of local PTP1B and/or calpain activities may prove beneficial in the treatment of impaired wound healing in diabetes. PMID: 27872190
    2. Studied the effect of cyclic uniaxial stretch (20%, 1 Hz), with and without the stimulation of vascular endothelial growth factor (VEGF), on sprouting angiogenesis by employing a stretchable three-dimensional cell culture model. PMID: 25376776
    3. Study shows that VEGFA mRNA in mammalian endothelial cells undergoes programmed translational readthrough (PTR) generating VEGF-Ax, an isoform containing a unique 22-amino-acid C terminus extension. PMID: 24949972
    4. Data indicate that superoxide dismutase (SOD) inhibited high glucose (HG)-induced expression of uPAR and VEGF in bovine retinal microvascular endothelial cell (REC). PMID: 23951261
    5. Exposure of endothelial cells to VEGF, high glucose, or hydrogen peroxide up-regulated the XBP1/IRE1 alpha and ATF6 arms of the unfolded protein response compared with untreated cells. PMID: 23395094
    6. Microvascular endothelial cells are more susceptible than aortic cells to advanced glycation end products-enhanced permeability and that AGE-enhanced permeability is dependent on VEGF expression induced by reactive oxygen species. PMID: 21296593
    7. VEGF supports germ cell survival and sperm production in bulls. PMID: 19633133
    8. analysis of polymorphisms of bovine VEGF gene and their associations with growth traits in Chinese cattle PMID: 20376703
    9. VEGF mRNA expression at estrus was higher than at the early I, early II and late luteal stages (P<0.05), whereas VEGF protein content was greatest at the early I luteal stage and decreased thereafter PMID: 20035107
    10. Alterations in the expression of VEGF-A and bFGF systems suggest that angiogenic factors are involved in abnormal placental development in cloned gestations, contributing to impaired fetal development and poor survival rates. PMID: 20198587
    11. Advanced glycation end products increase, through a protein kinase C-dependent pathway, expression in reignal endothelial cells; inhibited by gliclazide. PMID: 12126787
    12. VEGF signaling is regulated by atrial natriuretic peptide, which preserves endothelial cell tight junction functional morphology PMID: 12213803
    13. Airline pilots are good candidates to see if mutation in the VEGF promoter are responsible for some cases of amyotrophic lateral sclerosis. PMID: 12448348
    14. role in dowregulating Flk-1/KDR involves Cbl-mediated ubiquitination PMID: 12649282
    15. interaction with glucose-6-phosphate dehydrogenase is involved in angiogenesis PMID: 12777375
    16. Results suggest that leptin might elicit angiogenesis through vascular endothelial growth factor induction as well as pigment epithelium-derived factor suppression in pericytes. PMID: 12779248
    17. Shear stress and VEGF converge at the membrane receptor Flk-1 and these stimuli share the Flk-1/Cbl/Akt pathway in activating IKK activation. PMID: 14551058
    18. Tumor cells activate confluent, quiescent EC, promoting survival, phenotypic, and gene expression changes. Of importance, VEGF antagonism converts tumor-conditioned medium from protective to endothelial cell-damaging effects. PMID: 15075216
    19. MGP plays a role in endothelial cell function, by increasing transforming growth factor-beta1 activity and stimulating VEGF expression PMID: 15456771
    20. placenta growth factor expression is regulated by both VEGF and hyperglycaemia via VEGFR-2 PMID: 15501243
    21. VEGF has a role in increasing paracellular transport PMID: 15501249
    22. Thrombospondin-1 and -2 were coordinately expressed in the extravascular compartment of the ovary during early follicle development. VEGF was inversely expressed, with expression increasing as follicles developed. PMID: 15616224
    23. the activation of VEGFR-1 and VEGFR-2 heterodimer (VEGFR-1/R-2) is essential for PGI(2) synthesis mediated by VEGF-A(165) and VEGF-A(121), which cannot be reproduced by the parallel activation of VEGFR-1 and VEGFR-2 homodimers with corresponding agonists PMID: 15637071
    24. Reciprocally, adenovirus-mediated overexpression of Cyr61 in cells cultured under static conditions increased the expression of VEGF, alpha(v)-integrin, and SM alpha-actin. PMID: 15649872
    25. peroxynitrite causes increased expression of VEGF in vascular endothelial cells by a process that requires the activation of STAT3 PMID: 16257644
    26. ROS induce expression not only of VEGF but also of VEGFR2. VEGFR2 increase by ROS is mainly driven through a NF-kappaB-dependent pathway. PMID: 16899768
    27. expression of VEGF isoforms may be associated with characterization of the preovulatory phenotype during follicle development in the bovine ovary PMID: 17043385
    28. Gab1 is a novel critical regulatory component of endothelial cell migration and capillary formation with a key role in the activation of VEGF-evoked signaling pathways required for angiogenesis PMID: 17178724
    29. The results provide support for a role for VEGF in the primary to secondary follicle transition. PMID: 17290425
    30. This study reports the temporal changes in vascular endothelial growth factor A (VEGFA), fibroblast growth factor 2 (FGF2) and osteonectin during the follicular-luteal transition and corpus luteum development in the cow. PMID: 17360962
    31. a new molecular cascade, operating in retinal bovine pericytes and involving PKCbetaII, the mRNA-stabilizing protein HuR. and VEGF. In particular we show that PKCbetaII activation is responsible, through the RNA-binding protein HuR PMID: 18206386
    32. bFGF and VEGF control the corpus luteum formation and function. PMID: 18213648
    33. VEGF has an effect on the expression of its own transcription factor, HIF-1, and on VEGF itself PMID: 18305118
    34. By regulating VEGFR2 expression and activation, PKC-epsilon expression is critical for activation of Akt and eNOS by VEGF and contributes to VEGF-stimulated Erk activation, whereas PKC-alpha has opposite effects. PMID: 18323518
    35. Angiopoietin-2 may support the angiogenic effects of VEGF-A in these corpus luteum stages. PMID: 18541291
    36. DNA methylation of VEGF in blg(+/-) was noticeably decreased. PMID: 18789616
    37. VEGF-dependent production of NO by eNOS is mediated by as cascade involving ezrin, calpain, PI-3-kinase, AMPK and AKT. PMID: 19038867
    38. ALK1 and ALK5 are both essential for correct regulation of VEGF, and that disruption of either pathway leads to disease. PMID: 19506300
    39. inhibition of NO production by Ang-1, via phosphorylation of eNOS on Thr(497) by PKC zeta, is responsible, at least in part, for inhibition of VEGF-stimulated endothelial permeability by Ang-1. PMID: 19564638

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  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    PDGF/VEGF growth factor family
  • 數(shù)據(jù)庫鏈接:

    KEGG: bta:281572

    STRING: 9913.ENSBTAP00000056005

    UniGene: Bt.4138



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