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Bovine Thyroid Stimulating Hormone(TSH) ELISA Kit

  • 中文名稱:
    牛促甲狀腺素(TSH)酶聯免疫試劑盒
  • 貨號:
    CSB-E17548B
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    牛促甲狀腺素(TSH)酶聯免疫試劑盒(CSB-E17548B)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的TSH含量。TSH即促甲狀腺激素,是腺垂體分泌的促進甲狀腺生長和機能的激素。其可調控甲狀腺細胞的增殖與甲狀腺血液供應。研究多圍繞其與甲狀腺疾病如甲亢、甲減的關系展開,明確其作用機制有助于疾病的診斷、治療及病情監測。試劑盒檢測范圍為0.9 uIU/mL-60 uIU/mL,適用于牛甲狀腺相關疾病機制研究、藥物干預效果評估或畜牧養殖中生理狀態監測等科研場景本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    TSHB ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
  • 縮寫:
    TSH
  • Uniprot No.:
  • 種屬:
    Bos taurus (Bovine)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.9 uIU/mL-60 uIU/mL
  • 靈敏度:
    0.225 μIU/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %92-101
    1:2Average %88
    Range %84-92
    1:4Average %90
    Range %85-98
    1:8Average %92
    Range %88-96
  • 回收率:
    The recovery of bovine TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10095-104
    EDTA plasma (n=4)8480-88
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    µIU/ml OD1OD2AverageCorrected
    601.815 1.731 1.773 1.626
    301.279 1.237 1.258 1.111
    150.934 0.927 0.931 0.784
    7.50.623 0.671 0.647 0.500
    3.750.408 0.379 0.394 0.247
    1.80.315 0.297 0.306 0.159
    0.90.201 0.195 0.198 0.051
    00.150 0.143 0.147
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Indispensable for the control of thyroid structure and metabolism.
  • 基因功能參考文獻:
    1. Nitric oxide decreases TSH-induced FoxE1 expression, thus repressing the transcripcional activation of TPO gene. PMID: 26610751
    2. identification for the first time that ADAM10 is a TSHR cleavage enzyme and demonstration that TSH regulates its activation PMID: 18074395
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Glycoprotein hormones subunit beta family
  • 數據庫鏈接:

    KEGG: bta:281552

    STRING: 9913.ENSBTAP00000008264

    UniGene: Bt.475



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