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Bovine Selenoprotein P(SEPP1) ELISA kit

  • 中文名稱:
    牛硒蛋白P(SEPP1)酶聯免疫試劑盒
  • 貨號:
    CSB-EL021018BO
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    牛硒蛋白P(SEPP1)酶聯免疫試劑盒(CSB-EL021018BO)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的SEPP1含量。SEPP1即硒蛋白P,在人體硒穩態維持和抗氧化防御等方面發揮重要作用。其研究機制涉及運輸硒到各組織,還參與細胞內的抗氧化過程,減輕氧化應激。當前圍繞它在神經系統、心血管系統疾病及癌癥等方面的作用也有諸多研究。試劑盒檢測范圍為10 ng/mL-160 ng/mL,該產品可廣泛應用于動物營養學研究中的硒元素吸收評估、氧化應激相關疾病模型的生物標志物分析,或環境毒理學中重金屬暴露對硒蛋白代謝影響的探索,為畜牧健康管理及基礎醫學研究提供可靠的數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    SELENOP ELISA Kit; Selenoprotein P ELISA Kit; SeP ELISA Kit; Selenoprotein P-like protein ELISA Kit
  • 縮寫:
    SEPP1
  • Uniprot No.:
  • 種屬:
    Bos taurus (Bovine)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    10 ng/mL-160 ng/mL
  • 靈敏度:
    5 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine SEPP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:100 Average % 89
    Range % 86-102
    1:200 Average % 97
    Range % 91-105
    1:400 Average % 93
    Range % 88-100
    1:800 Average % 91
    Range % 87-96
  • 回收率:
    The recovery of bovine SEPP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 86-99
    EDTA plasma (n=4) 93 85-102
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average  
    160 0.105 0.102 0.104  
    80 0.196 0.189 0.193  
    40 0.321 0.332 0.327  
    20 0.622 0.608 0.615  
    10 1.098 1.108 1.103  
    0 2.193 2.216 2.205  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Constitutes a major selenium pool in the brain and may play an important role in developing and/or modulating the morphology of neurons and/or glial cells.
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Selenoprotein P family
  • 組織特異性:
    Brain and kidney. Most prominently expressed in the cerebellar cortex, hippocampus and olfactory bulb.
  • 數據庫鏈接:

    KEGG: bta:282066

    UniGene: Bt.64610



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