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Bovine Pregnancy Specific Protein B(PSPB)ELISA Kit

  • 中文名稱:
    牛妊娠特異性蛋白B(PSPB)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E13353B
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    牛妊娠特異性蛋白B(PSPB)酶聯(lián)免疫試劑盒(CSB-E13353B)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的PAG1含量。PAG1是重要靶點(diǎn)。它在細(xì)胞信號傳導(dǎo)等方面發(fā)揮作用,與細(xì)胞生長、增殖等生理過程相關(guān)。研究其機(jī)制有助于了解疾病發(fā)生發(fā)展,可能為腫瘤等疾病的治療提供新思路,通過調(diào)控PAG1有望開發(fā)出新型治療手段。試劑盒檢測范圍為0.06 ng/mL-24 ng/mL,本產(chǎn)品適用于畜牧科研中妊娠狀態(tài)監(jiān)測、胎盤功能評估、生殖內(nèi)分泌研究及動物繁殖性能分析等場景,其寬泛的樣本兼容性可滿足血清/血漿直接檢測及組織樣本經(jīng)勻漿處理后的定量需求。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Pregnancy-associated glycoprotein 1 ELISA Kit; PAG 1 ELISA Kit; EC 3.4.23.- ELISA Kit; Pregnancy-specific protein B ELISA Kit; PSP-B ELISA Kit
  • 縮寫:
    PAG1
  • Uniprot No.:
  • 種屬:
    Bos taurus (Bovine)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.06 ng/mL-24 ng/mL
  • 靈敏度:
    0.06 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine PSPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 90-104  
    1:2 Average % 94  
    Range % 88-105  
    1:4 Average % 90  
    Range % 85-96  
    1:8 Average % 97  
    Range % 89-105  
  • 回收率:
    The recovery of bovine PSPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 84-94  
    EDTA plasma (n=4) 97 90-105  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    24 0.155 0.146 0.151    
    4.5 0.344 0.332 0.338    
    0.75 0.571 0.582 0.577    
    0.21 1.006 0.998 1.002    
    0.06 1.562 1.575 1.569    
    0 2.191 2.223 2.207    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能:
    Appears to be proteolytically inactive.
  • 基因功能參考文獻(xiàn):
    1. This study examines expression patterns of the genes SERPINA14, PAG, and PAG2 at the fetal-maternal interface in dairy heifers experimentally infected with N caninum during the second trimester of pregnancy, when most abortions takes place in natural conditions. PMID: 27045629
    2. The clinical implications of these findings are that following infection with N. caninum, most cows will experience some level of placental damage and that this injury correlates with foetal fluid PAG levels and pH. PMID: 26936628
    3. These findings indicate that IFN-gamma production correlates negatively and the production of antibodies against N. caninum is uncorrelated with plasma pregnancy-associated glycoproteins levels. PMID: 24456132
    4. Restraint stress for 2 h during early pregnancy in heifers increased blood cortisol, but it did not affect the concentrations of progesterone and PAG-1 between days 30 to 40 of gestation. PMID: 21872319
  • 亞細(xì)胞定位:
    Secreted, extracellular space.
  • 蛋白家族:
    Peptidase A1 family
  • 組織特異性:
    Trophoblast and placental tissue. Produced specifically in the invasive binucleate cells of the placenta. Becomes detectable in maternal serum soon after implantation.
  • 數(shù)據(jù)庫鏈接:


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