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Bovine Major prion protein(PRNP) ELISA kit

  • 中文名稱:
    牛主要朊病毒蛋白(PRNP)酶聯免疫試劑盒
  • 貨號:
    CSB-EL018739BO
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    牛主要朊病毒蛋白(PRNP)酶聯免疫試劑盒(CSB-EL018739BO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的PRNP含量。PRNP 編碼的蛋白為朊蛋白,在神經系統等中發揮重要作用。其突變或異常聚集可引發朊蛋白病,如瘋牛病、克雅氏病等。研究機制主要聚焦于其正常功能維持、異常折疊機制及傳播途徑,以尋找診斷和治療方法。試劑盒檢測范圍為0.01 ng/ml-10 ng/ml,適用于科研場景中PRNP蛋白表達調控、病原體感染機制研究、疫苗開發過程中的生物標志物檢測等;為神經退行性疾病模型構建、畜牧業病原體篩查、抗病毒藥物篩選等基礎研究提供可靠工具本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    PRNP ELISA Kit; PRP ELISA Kit; Major prion protein ELISA Kit; PrP ELISA Kit; Major scrapie-associated fibril protein 1 ELISA Kit; CD antigen CD230 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Bos taurus (Bovine)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    0.01 ng/ml-10 ng/ml
  • 靈敏度:
    0.01 ng/ml
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of bovine PRNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    93

    Range %

    85-102

    1:2

    Average %

    92

    Range %

    86-105

    1:4

    Average %

    88

    Range %

    82-98

    1:8

    Average %

    93

    Range %

    88-102

  • 回收率:

    The recovery of bovine PRNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    92

    85-100

    EDTA plasma (n=4)

    98

    93-102

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    10

    2.603

    2.546

    2.575

    2.399

    3.33

    1.906

    1.933

    1.920

    1.744

    1.11

    1.336

    1.309

    1.323

    1.147

    0.37

    0.871

    0.895

    0.883

    0.707

    0.12

    0.564

    0.595

    0.580

    0.404

    0.04

    0.380

    0.362

    0.371

    0.195

    0.01

    0.266

    0.247

    0.257

    0.081

    0

    0.171

    0.180

    0.176

     

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains.
  • 基因功能參考文獻:
    1. Data suggest that the E211K prion protein provides the opportunity for future analysis of physiological changes over time. PMID: 29262866
    2. Disparate Modes of Evolution Shaped Modern Prion (PRNP) and Prion-Related Doppel (PRND) Variation in Domestic Cattle PMID: 27224046
    3. the data indicate a four-rung beta-solenoid structure as a key feature for the architecture of infectious mammalian prions. PMID: 27606840
    4. Insights into Chronic Wasting Disease and Bovine Spongiform Encephalopathy Species Barriers by Use of Real-Time Conversion PMID: 26157118
    5. Misfolded structures, with nonnative beta-strands formed in the flexible N-terminal domain of PRNP were found in acidic pH simulations. PMID: 24970211
    6. This work demonstrates that this isolate is transmissible, has a BSE-H phenotype when transmitted to cattle with the K211 polymorphism, and has molecular features that distinguish it from other cases of BSE-H described in the literature. PMID: 22715405
    7. Genetic characterization of PRNP promoter indel variations and the polymorphism of open reading frames (ORFs) of PRNP and bovine prion-like Shadoo (SPRN) genes, are reported. PMID: 22723200
    8. data showed a differential timing of PrPC expression during early bovine development; the cell-specific expression of PrPC in bovine embryos was revealed to included the developing brain and spinal cord, peripheral nervous system, liver, and mesonephros PMID: 22674901
    9. The centripetal spread of bovine spongiform encephalopathy prions along the autonomic nervous system to the central nervous system, starting already halfway in the incubation time, is reported. PMID: 22781833
    10. The results indicate that certain negative feedback response elements are located in the 5' flanking region and intron1 of the PRNP gene, suggesting that regulation by transcription factors such as Sp1 and RP58 may contribute to the negative feedback mechanism of PRNP. PMID: 22412936
    11. allele and haplotype segregation at the polymorphic sites within the promoter (23indel) and intron 1 (12indel) regions of the PRNP PMID: 22258312
    12. PRNP gene variation in Pakistani cattle and buffaloes. PMID: 22634099
    13. The Japanese and Canadian L-type bovine spongiform encephalopathy prions are identical to those from the European cases. PMID: 22453183
    14. A significant relation between the investigated PRNP indel polymorphisms (23 and 12 bp indels), and susceptibility of Polish Holstein-Friesian cattle to classical bovine spongiform encephalopathy, is reported. PMID: 22170597
    15. fibrils formed by the rabbit protein contain less beta-sheet structure and more alpha-helix structure than those formed by the proteins from human and cow PMID: 21943430
    16. these results identify a novel PrP(C)-interacting protein KCTD1 and suggest a new approach to investigating the unidentified physiological cellular function of PrP(C). PMID: 22138399
    17. Different overall sensitivities of prion protein toward urea denaturation occurs with stabilities in the following species order: hamster PMID: 21800884
    18. The polymorphisms of PRNP gene, including SNP in exon 3, 23-bp indel in promoter region, 12-bp indel in intron 1 in 2 Chinese indigenous cattle breeds of northeast China, were investigated. PMID: 21120616
    19. The del/del genotype or at least its del allele may modulate the expression of PRNP at the 23-bp locus in the medulla oblongata of the cattle breeds studied. PMID: 21611160
    20. PRNP haplotype is associated with classical bovine spongiform encephalopathy incidence in European Holstein cattle. PMID: 20862290
    21. The present study demonstrated that vector-based siRNA expression systems is an efficient approach to knockdown the PRNP gene expression in bovine fibroblast cells. PMID: 19821149
    22. The interaction of the molecular chaperone Hsc70 (HSPA8) with recombinant PrP was investigated. PMID: 20434583
    23. Role the of regulatory domains within intron 1 was defied in regulating the promoter activity of the bovine prion protein gene. PMID: 20553871
    24. unusual conformational plasticity of prion protein PMID: 20380155
    25. PRNP polymorphisms in native Chinese cattle are reportd. PMID: 20140032
    26. Study found a significant correlation between promoter indel polymorphisms and PRNP-mRNA expression; also report a novel difference in PRNP expression between Japanese Black and Japanese Brown cattle breeds. PMID: 19917050
    27. existence of two 2'-fluoro-RNA aptamer binding sites within the PrP molecule PMID: 12902353
    28. a 23-bp insertion/deletion polymorphism in the putative PRNP promoter region shows a significant association with bovine spongiform encephalopathy susceptibility in our animals. PMID: 14727152
    29. Transgenic mice expressing bovine PrP (boTg mice) bearing an additional octapeptide insertion to the wild type (seven octapeptide repeats instead of six) showed an altered course of bovine spongiform encephalopathy (BSE) infection PMID: 14999066
    30. PRNP shows no direct CAGA box correspondence with the APP superfamily members. PMID: 15208260
    31. evaluated the patterns of nucleotide variation associated with PRNP exon 3 for 36 breeds of domestic cattle PMID: 15477588
    32. A functional promoter analysis of the different bovine PRNP promoter alleles by reporter gene assays in vitro and PRNP mRNA levels in calves with different PRNP genotypes in vivo are reported. PMID: 16141216
    33. the genomic structure of three genes, PRNP, PRND, and RASSF2, within the syntenic region of the bovine genome is highly conserved in order and orientation. PMID: 16460908
    34. J protein family serves as a 'folding catalyst' for prion protein and implicates Rdj2 as a factor in the protection against prion diseases PMID: 16774738
    35. 10 single nucleotide polymorphisms (SNPs) were discovered in the coding region of the Caracu PRNP gene. PMID: 16965405
    36. DNA variants in the PRNP gene are associated with genetic disposition to bovine spongiform encephalopathy. PMID: 16978176
    37. results demonstrate a substantial genetic PRNP associated component for BSE susceptibility in cattle PMID: 17014722
    38. data indicate that bovine spongiform encephalopathy agent can be altered by crossing a species barrier, raising concerns about the virulence of this new prion towards other species, including humans. PMID: 17079295
    39. The number of polymorphisms in the prion gene region of U.S. cattle is nearly four times greater than previously described; these polymorphisms define PRNP haplotypes that may influence BSE susceptibility in cattle PMID: 17092337
    40. Immunohistochemical characterization of cell types expressing the cellular prion protein in the small intestine was studied and compared to the mouse. PMID: 17165097
    41. identified the deletion/insertion polymorphism of the prion protein gene (PRNP) within the promoter sequence (23 bp), intron 1 (12 bp) and 3' untranslated region (14 bp) PMID: 17272863
    42. These results advocate that certain polymorphisms such as specific protein 1 binding site polymorphisms in the bovine PRNP promoter region in Japanese Black cattle could influence promoter activity. PMID: 17283444
    43. Genotype and allele frequencies of the 23 bp indel polymorphism in Korean Holsteins cattle. PMID: 17426768
    44. polymorphisms in the PRNP promoter region have an influence on susceptibility to BSE PMID: 17437640
    45. Levels of the protein for PrP are higher in theca cells of dominant compared to subordinate follicles. PMID: 17595008
    46. Results describe the expression of the prion protein gene (PRNP) and cellular prion protein (PrPc) in cattle and sheep fetuses and maternal tissues during pregnancy. PMID: 17605301
    47. no association between genotype and resistance to atypical bovine spongiform encephalopathy or experimentally inoculated transmissible spongiform encephalopathies. PMID: 17709775
    48. PrP(C) specifically binds tetraspanin-7 through the region corresponding to bovine PrP(154-182) containing alpha-helix 1. PMID: 17981143
    49. The results suggest that Octapeptide repeat region of PrP(C) is required for the early step of efficient PrP(Sc) production. PMID: 17981146
    50. Polymorphisms of Two Indels at the PRNP Gene in Three Beef Cattle Herds PMID: 18064562

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  • 相關疾?。?/div>
    Variations in PRNP are responsible of transmissible bovine spongiform encephalopathies (BSE), a class of neurodegenerative diseases that affect various mammals. These diseases are caused by abnormally folded prion proteins. BSE can be subdivided into at least three groups: classical, H-type and L-type, with the latter 2 collectively referred to as atypical BSE. Susceptibility or resistance to a BSE disease can be influenced by at least 3 factors related to the host prion protein: protein expression levels, number of octapeptide repeats, and specific polymorphisms. In cattle, as in humans, BSEs can occur as infectious, spontaneous and genetic diseases.
  • 亞細胞定位:
    Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus.
  • 蛋白家族:
    Prion family
  • 數據庫鏈接:

    STRING: 9913.ENSBTAP00000043233

    UniGene: Bt.4737



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