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  4. Bovine Interleukin 2,IL-2 ELISA Kit

Bovine Interleukin 2,IL-2 ELISA Kit

  • 中文名稱：
    牛白介素2(IL-2)酶聯免疫試劑盒
  • 貨號：
    CSB-E09810b
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：
    牛白介素2(IL-2)酶聯免疫試劑盒（CSB-E09810b）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL2含量。IL2是重要的細胞因子，可調節免疫細胞生長、增殖和分化。它在免疫系統激活和維持免疫平衡方面作用關鍵。其研究機制主要圍繞調控T細胞和NK細胞功能，在腫瘤免疫治療、自身免疫性疾病等領域是研究熱門靶點。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于科研領域探究牛類免疫機制、疫苗效力評估、畜牧病原感染模型中的細胞免疫應答分析，以及炎癥性疾病或免疫抑制相關研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    IL2 ELISA kit; IL-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %81-90
    1:2Average %100
    Range %97-103
    1:4Average %101
    Range %98-105
    1:8Average %94
    Range %86-98
  • 回收率：
    The recovery of bovine IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-97
    EDTA plasma (n=4)9692-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.151 2.099 2.125 2.040
    10001.683 1.656 1.670 1.585
    5001.284 1.270 1.277 1.192
    2500.846 0.833 0.840 0.755
    1250.502 0.498 0.500 0.415
    62.50.367 0.356 0.362 0.277
    31.250.221 0.213 0.217 0.132
    00.086 0.084 0.085 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • 基因功能參考文獻：
    1. Pretreatment of neonatal PBMC with IL-1beta, TNF-alpha or IFN-gamma promotes mitogenic response to ConA through up-regulating the production of IL-2 and the expression of the mature IL-2 receptor. PMID: 12906107
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    IL-2 family
  • 數據庫鏈接：

    KEGG: bta:280822

    STRING: 9913.ENSBTAP00000027819

    UniGene: Bt.341



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