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Bovine Interleukin-18(IL18) ELISA kit

  • 中文名稱:
    牛白介素18(IL18)酶聯免疫試劑盒
  • 貨號:
    CSB-EL011608BO
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    牛白介素18(IL18)酶聯免疫試劑盒(CSB-EL011608BO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IL18含量。IL-18是一種促炎細胞因子,在先天性和適應性免疫反應中發揮關鍵作用。它可誘導Th1細胞產生IFN-γ,參與免疫調節。多個研究表明,IL-18在多種炎癥性疾病、自身免疫病和腫瘤的發病機制中起重要作用,是極具潛力的治療靶點。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL,適用于研究牛感染性疾病模型(如病毒或細菌感染)、免疫調節機制探索、疫苗效力評估等科研場景,也可用于評估營養干預或環境應激對牛免疫狀態的影響。通過標準化操作流程,研究者可快速獲得IL18在免疫相關研究中的動態變化數據,為深入解析牛類免疫系統功能提供技術支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Bos taurus (Bovine)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    31.25 pg/mL-2000 pg/mL
  • 靈敏度:
    7.81 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 88
    Range % 83-92
    1:2 Average % 97
    Range % 91-105
    1:4 Average % 95
    Range % 92-99
    1:8 Average % 93
    Range % 87-98
  • 回收率:
    The recovery of bovine IL18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 90-110
    EDTA plasma (n=4) 97 90-105
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 3.011 2.978 2.995 2.900
    1000 2.511 2.522 2.517 2.422
    500 1.496 1.504 1.500 1.405
    250 0.705 0.716 0.711 0.616
    125 0.382 0.375 0.379 0.284
    62.5 0.226 0.234 0.230 0.135
    31.25 0.152 0.159 0.156 0.061
    0 0.093 0.097 0.095  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.
  • 基因功能參考文獻:
    1. These results suggest that BAPC-1 is a stem/progenitor cell line and modulates the immuno-endocrine function of the anterior pituitary cells through its production of interleukin-18 and the IL-18 receptor. PMID: 18761716
  • 亞細胞定位:
    Cytoplasm. Secreted.
  • 蛋白家族:
    IL-1 family
  • 數據庫鏈接:

    KEGG: bta:281249

    STRING: 9913.ENSBTAP00000055793

    UniGene: Bt.234



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