Bovine Interferon α,IFN-α ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

牛α干擾素(IFN-α)酶聯(lián)免疫試劑盒（CSB-E13726B）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IFN-α含量。IFN-α即α-干擾素，是機(jī)體受病毒感染等刺激后，由白細(xì)胞產(chǎn)生的細(xì)胞因子。其具有抗病毒、抗腫瘤和免疫調(diào)節(jié)等作用。研究機(jī)制主要是與細(xì)胞表面受體結(jié)合，激活信號通路，誘導(dǎo)多種抗病毒蛋白產(chǎn)生，抑制病毒復(fù)制并調(diào)節(jié)免疫細(xì)胞活性。試劑盒檢測范圍為25 pg/mL-1000 pg/mL，適用于畜牧獸醫(yī)研究、抗病毒藥物開發(fā)等科研領(lǐng)域，如牛病毒性腹瀉（BVDV）、口蹄疫等病毒感染模型的免疫機(jī)制研究，或疫苗免疫后干擾素水平動態(tài)監(jiān)測等實(shí)驗(yàn)，為探索IFN-α在抗病毒免疫中的功能提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Interferon alpha-1 ELISA Kit
縮寫：

IFN-α
Uniprot No.：

P07348
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma
檢測范圍：

25 pg/mL-1000 pg/mL
靈敏度：

25 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-101
1:2	Average %	91
	Range %	85-98
1:4	Average %	95
	Range %	90-103
1:8	Average %	96
	Range %	90-102

回收率：

The recovery of bovine IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	97	91-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
亞細(xì)胞定位：

Secreted.
蛋白家族：

Alpha/beta interferon family
數(shù)據(jù)庫鏈接：

STRING: 9913.ENSBTAP00000055837