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  4. Bovine Haptoglobin,Hpt/HP ELISA KIT

Bovine Haptoglobin,Hpt/HP ELISA KIT

  • 中文名稱：
    牛結合珠蛋白/觸珠蛋白(Hpt/HP)酶聯免疫試劑盒
  • 貨號：
    CSB-E08585b
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產品詳情

  • 產品描述：
    牛結合珠蛋白/觸珠蛋白(Hpt/HP)酶聯免疫試劑盒（CSB-E08585b）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的HP含量。牛結合珠蛋白（Haptoglobin，Hp）是一種在哺乳動物血清中廣泛存在的酸性糖蛋白，主要功能是與游離血紅蛋白結合，引導其進入降解代謝程序，防止其對機體的損傷。其亞型與多種疾病如感染、糖尿病、腫瘤等存在相關性，具有作為治療藥物的潛力。牛結合珠蛋白的分離純化方法包括硫酸銨沉淀、聚乙二醇沉淀等，這些方法有助于大規模純化結合珠蛋白，為后續的藥物開發提供材料。試劑盒檢測范圍為7.81 ng/mL-2000 ng/mL，該產品適用于畜牧科研中炎癥相關疾病的機制研究、疫苗或藥物干預效果評估，以及動物健康監測等場景，為牛群疾病模型建立、營養代謝研究提供關鍵數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    HPHaptoglobin [Cleaved into: Haptoglobin alpha chain; Haptoglobin beta chain] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    7.81 ng/mL-2000 ng/mL
  • 靈敏度：
    7.81 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine Hpt in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1000 Average % 84  
    Range % 80-88  
    1:2000 Average % 90  
    Range % 85-94  
    1:4000 Average % 103  
    Range % 99-112  
    1:8000 Average % 98  
    Range % 94-102  
  • 回收率：
    The recovery of bovine Hpt spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-99  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    2000 0.243 0.252 0.248    
    500 0.412 0.436 0.424    
    120 0.793 0.754 0.774    
    31.25 1.214 1.239 1.227    
    7.81 1.795 1.724 1.760    
    0 2.405 2.359 2.382    
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

相關問答

■ 常見問題解答
Q:

a. Is the capture antibody a monoclonal or polyclonal?
b. What is the host of the capture antibody?
c. Is the detection antibody a monoclonal or polyclonal?
d. What is the host of the detection antibody?
e. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
f. If recombinant protein, is it full length or partial?
g. What is the species of the standard– human or something else?

A:
Thanks for your inquiry.
The antibody used in the kit is rabbit polyclonal antibody.
The standard is purified crude extract of native samples.

靶點詳情

  • 功能：
    As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
  • 基因功能參考文獻：
    1. These results indicate that it is possible to monitor the course of pyometra in cows based on the evaluation of the concentration of cytokines and Hp in the serum and uterine washings. PMID: 25704413
    2. These data demonstrate that serum neutrophil haptoglobin-MMP 9 complexes appear sooner and decline more rapidly than other acute phase proteins. PMID: 25358728
    3. An increase in serum amyloid A or haptoglobin in dairy cows with abomasal displacementis not specific for inflammation or bacterial infection; increased levels of either may indicate the presence of hepatic lipidosis. PMID: 20391639
    4. Haptoglobin concentration may be a useful indicator for cows with postpartum reproductive disorders. PMID: 14960809
    5. Presence in the milk of the acute phase proteins (APP), haptoglobin (Hp) and serum amyloid A to see the relationships in cow composite and bulk tank milk samples. PMID: 17227596
    6. Bovine granulocytes, isolated from peripheral blood of healthy cattle, contain abundant amounts of haptoglobin within the granules. PMID: 17681384
    7. This study suggests that neutrophils and epithelial cells may play an essential role in elevating milk haptoglobin (Hp) in addition to previous suggestions that Hp may be derived from mammary tissues and circulation. PMID: 19094922

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  • 亞細胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Peptidase S1 family
  • 組織特異性：
    Expressed by the liver and secreted in plasma.
  • 數據庫鏈接：


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