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  4. Bovine Fibronectin,FN ELISA Kit

Bovine Fibronectin,FN ELISA Kit

  • 中文名稱(chēng)：
    牛纖連蛋白(FN)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13781B
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛纖連蛋白(FN)酶聯(lián)免疫試劑盒（CSB-E13781B）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿、細(xì)胞裂解物樣本中的FN1含量。FN1即纖連蛋白 1，是細(xì)胞外基質(zhì)的重要成分，在細(xì)胞黏附、遷移、增殖等過(guò)程中發(fā)揮關(guān)鍵作用。研究顯示，其異常表達(dá)與腫瘤的發(fā)生、發(fā)展及轉(zhuǎn)移密切相關(guān)。深入探究 FN1 的作用機(jī)制，有望為腫瘤等疾病的治療提供新靶點(diǎn)和思路。試劑盒檢測(cè)范圍為0.137 μg/mL-100 μg/mL，科研人員可將其應(yīng)用于細(xì)胞外基質(zhì)功能研究、細(xì)胞粘附機(jī)制探索、組織再生模型構(gòu)建或炎癥相關(guān)疾病模型的分子機(jī)制分析，例如評(píng)估體外培養(yǎng)細(xì)胞分泌FN的水平，或分析特定病理狀態(tài)下組織微環(huán)境中FN的動(dòng)態(tài)變化。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    FN1Fibronectin ELISA Kit; FN) [Cleaved into: Anastellin] ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類(lèi)型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    0.137 μg/mL-100 μg/mL
  • 靈敏度：
    0.137 μg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:5 Average % 92    
    Range % 85-102    
    1:10 Average % 97    
    Range % 91-110    
    1:20 Average % 89    
    Range % 83-96    
    1:40 Average % 95    
    Range % 84-100    
  • 回收率：
    The recovery of bovine FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 96 88-105    
    EDTA plasma (n=4) 97 92-105    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    µg/ml OD1 OD2 Average Corrected    
    100 1.839 1.803 1.821 1.677    
    33 1.657 1.649 1.653 1.509    
    11 1.451 1.435 1.443 1.299    
    3.7 0.958 0.992 0.975 0.831    
    1.2 0.643 0.667 0.655 0.511    
    0.4 0.372 0.354 0.363 0.219    
    0.13 0.251 0.243 0.247 0.103    
    0 0.145 0.142 0.144 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.; Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • 亞細(xì)胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 組織特異性：
    Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
  • 數(shù)據(jù)庫(kù)鏈接：


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