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Your Good Partner in Biology Research

Bovine Fibrinogen,Fb Elisa Kit

  • 中文名稱:
    牛纖維蛋白原(Fb)酶聯免疫試劑盒
  • 貨號:
    CSB-E13283B
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    牛纖維蛋白原(Fb)酶聯免疫試劑盒(CSB-E13283B)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的Fb含量。試劑盒檢測范圍為3.125 μg/mL-200 μg/mL,本產品適用于動物醫學研究、畜牧業健康監測、營養代謝評估等科研領域,特別針對凝血功能異常模型或炎癥相關研究中的纖維蛋白原動態變化分析,為科研人員提供穩定、可重復的檢測方案。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    N/A
  • 縮寫:
    Fb
  • 種屬:
    Bos taurus (Bovine)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    3.125 μg/mL-200 μg/mL
  • 靈敏度:
    1.56 μg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine Fb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 90  
    Range % 86-97  
    1:40 Average % 96  
    Range % 92-100  
    1:80 Average % 85  
    Range % 81-86  
    1:160 Average % 89  
    Range % 83-98  
  • 回收率:
    The recovery of bovine Fb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-95  
    EDTA plasma (n=4) 96 92-100  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ug/ml OD1 OD2 Average    
    200 0.196 0.185 0.191    
    100 0.313 0.322 0.318    
    50 0.484 0.489 0.487    
    25 0.733 0.763 0.748    
    12.5 1.022 1.104 1.063    
    6.25 1.338 1.420 1.379    
    3.12 1.549 1.578 1.564    
    0 2.125 2.163 2.144    
  • 數據處理:
  • 貨期:
    3-5 working days

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