Bovine Beta-Endorphin,β-EP ELISA Kit
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中文名稱:牛β內(nèi)啡肽(β-EP)酶聯(lián)免疫試劑盒
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貨號:CSB-E13051B
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規(guī)格:96T/48T
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價格:¥4200/¥3000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:牛β內(nèi)啡肽(β-EP)酶聯(lián)免疫試劑盒(CSB-E13051B)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的β-EP含量。β - EP即β - 內(nèi)啡肽,是人體自身產(chǎn)生的具有強大鎮(zhèn)痛作用的內(nèi)源性活性物質(zhì)。其研究機制主要圍繞它與體內(nèi)阿片受體結(jié)合,調(diào)節(jié)痛覺傳導(dǎo)、情緒等生理過程,在鎮(zhèn)痛、精神疾病等領(lǐng)域研究較多,對理解人體生理和開發(fā)相關(guān)療法有重要意義。試劑盒檢測范圍為46.88 pg/mL-3000 pg/mL,可為神經(jīng)生物學(xué)、疼痛機制研究或應(yīng)激反應(yīng)動物模型提供數(shù)據(jù)支持,例如探究藥物干預(yù)、環(huán)境刺激對β - EP表達水平的影響;適用于牛源樣本的體外定量分析。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:beta-Endorphin, beta-EP
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縮寫:β-EP
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種屬:Bos taurus (Bovine)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:46.88 pg/mL-3000 pg/mL
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靈敏度:11.72 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Cancer
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of bovine β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 90-99 1:2 Average % 84 Range % 80-88 1:4 Average % 92 Range % 88-97 1:8 Average % 90 Range % 85-94 -
回收率:
The recovery of bovine β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 99 95-104 EDTA plasma (n=4) 92 88-96 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 3000 1.701 1.612 1.657 1.559 1500 1.376 1.458 1.417 1.319 750 1.113 1.104 1.109 1.011 375 0.832 0.809 0.821 0.723 187.5 0.524 0.560 0.542 0.444 93.75 0.367 0.359 0.363 0.265 46.88 0.264 0.251 0.258 0.160 0 0.099 0.097 0.098 -
數(shù)據(jù)處理:
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貨期:3-5 working days
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