Bovine Apolipoprotein A-I(APOA1) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛載脂蛋白A1(APOA1)酶聯免疫試劑盒（CSB-EL001913BO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOA1含量。APOA1是重要的載脂蛋白，在脂質代謝中意義重大。研究顯示，其能促進膽固醇逆向轉運，抑制動脈粥樣硬化。還可穩定高密度脂蛋白結構，發揮抗炎癥、抗氧化作用。對其深入研究有望為心血管疾病等的防治提供新思路。試劑盒檢測范圍為78 ng/mL-5000 ng/mL，本試劑盒適用于探索牛脂質代謝相關疾病機制、評估營養干預對脂蛋白合成的影響，或作為動物模型中動脈粥樣硬化研究的工具，為畜牧養殖健康管理、動物疾病模型構建及基礎醫學研究提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

APOA1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit
縮寫：

APOA1
Uniprot No.：

P15497
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

78 ng/mL-5000 ng/mL
靈敏度：

19.5 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine APOA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	90
	Range %	87-94
1:2000	Average %	96
	Range %	91-100
1:4000	Average %	93
	Range %	87-97
1:8000	Average %	100
	Range %	96-103

回收率：

The recovery of bovine APOA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	82-87
EDTA plasma (n=4)	89	85-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
基因功能參考文獻：
1. the model of a two-step process for the transendothelial transport of apoA-I in which apoA-I is initially lipidated by ABCA1 and then further processed by ABCA1-independent mechanisms. PMID: 21209084
亞細胞定位：

Secreted.
蛋白家族：

Apolipoprotein A1/A4/E family
組織特異性：

Major protein of plasma HDL, also found in chylomicrons.
數據庫鏈接：

KEGG: bta:281631

STRING: 9913.ENSBTAP00000002914

UniGene: Bt.49157