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  4. Bovine α1-Acid glycoprotein,α1-AGP ELISA Kit

Bovine α1-Acid glycoprotein,α1-AGP ELISA Kit

  • 中文名稱：
    牛α1酸性糖蛋白(α1-AGP)酶聯免疫試劑盒
  • 貨號：
    CSB-E08579b
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產品詳情

  • 產品描述：
    牛α1酸性糖蛋白(α1-AGP)酶聯免疫試劑盒（CSB-E08579b）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ORM1含量。ORM1 即阿片樣物質 μ 型受體 1，是一種與阿片類物質作用相關的靶點。其背景上在痛覺調節等生理過程有重要地位。研究機制方面，主要圍繞其與配體結合，通過信號轉導通路發揮效應，在鎮痛藥物研發等領域是重要研究對象。試劑盒檢測范圍為62.5 ng/mL-4000 ng/mL，該產品適用于科研領域，如評估牛類感染性疾病模型、炎癥相關藥效學研究、營養代謝對免疫系統的影響，或通過組織勻漿分析特定器官在病理條件下的蛋白表達特征。為畜牧獸醫研究、動物健康監測及基礎醫學機制探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    ORM1 ELISA kit; AGPAlpha-1-acid glycoprotein ELISA kit; Orosomucoid ELISA kit; OMD ELISA kit
  • 縮寫：
    ORM1
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    62.5 ng/mL-4000 ng/mL
  • 靈敏度：
    15.6 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine α1-AGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1000 Average % 102  
    Range % 94-106  
    1:2000 Average % 92  
    Range % 84-97  
    1:4000 Average % 101  
    Range % 95-104  
    1:8000 Average % 97  
    Range % 92-101  
  • 回收率：
    The recovery of bovine α1-AGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 88-99  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    4000 0.229 0.225 0.227    
    2000 0.450 0.459 0.455    
    1000 0.698 0.656 0.677    
    500 1.016 0.987 1.002    
    250 1.264 1.243 1.254    
    125 1.572 1.514 1.543    
    62.5 1.671 1.634 1.653    
    0 1.815 1.789 1.802    
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
  • 基因功能參考文獻：
    1. The presence of lipopolysaccharide binding protein (LBP) and alpha1-acid glycoprotein (AGP) was demonstrated in all seven adipose tissue depots. Expression of AGP and LBP suggests that they may have roles as local and systemic inflammatory adipokines. PMID: 25542063
    2. results provide evidence that locally produced alpha-1 acid glycoprotein may be involved in protecting sperm from phagocytosis by polymorphonuclear neutrophils in the bovine oviduct PMID: 24931131
    3. Studied the regulation and immune function of AGP in cultured bovine oviduct epithelial cells. PMID: 25123565
    4. As in human AGP and several other lipocalins, a temperature-induced molten globule state has been observed in the denaturation pathway of bovine AGP. PMID: 24530968
    5. In the mitochondrial membrane, both AGP and MLQ proteins are almost quantitatively bound to ATP synthase. PMID: 17575325
    6. Alpha1-acid glycoprotein isoforms that can be detected in bovine milk are reported. PMID: 17692138
    7. study provides evidence that the expression of CD18 was downregulated by the plasma glycoforms of alpha-1 acid glycoprotein and inhibited the chemotaxis of monocytes PMID: 18577362

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 數據庫鏈接：


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